NAD+ Injection Dosage Guide: Reconstitution, Units, and Nasal Spray

Dosing Guide — 2026-11-10

NAD+ (nicotinamide adenine dinucleotide) is a longevity and cellular-energy research compound. The injectable vials require careful dosing — and a slow injection to avoid the characteristic flush. This guide covers reconstitution and units for the 500mg and 1000mg vials, the pulsed schedule, and the gentler nasal-spray option. The numbers match our NAD+ 500mg, NAD+ 1000mg, and NAD+ spray protocol pages.

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NAD+ 500mg

Standard reconstitution: add exactly 5 mL bacteriostatic water.

Metric	Value
Peptide	500 mg
Bac water	5 mL
Concentration	100 mg/mL
Per 10 units	10 mg
Per 50 units	50 mg

NAD+ 1000mg

Standard reconstitution: add exactly 10 mL bacteriostatic water (same 100 mg/mL — the 1000mg vial just lasts twice as long).

Metric	Value
Per 10 units	10 mg
Per 50 units	50 mg
Per 100 units	100 mg

Dosing

Schedule	Dose	Units	Frequency
Daily	25–50 mg	25–50 units	Once daily
Pulsed	50–100 mg	50–100 units	3× weekly (e.g. Mon/Wed/Fri)

Inject very slowly — about 30 seconds — to minimize the site burn / flush that NAD+ is known for.

Nasal spray (gentler, no mixing)

The NAD+ spray is pre-mixed and ready to use:

Conservative: 1 spray per nostril, once daily — start here.
Greater effect: 2 sprays per nostril.

Intranasal is a gentler, lower-dose route — more of a daily maintenance option than a high-dose loading protocol.

Cycling, side effects, and TMG

Cycle: 1–3 months common, 2–4 weeks off.
Side effects: site flushing/burning (inject slowly); occasional mild transient headache or chest pressure.
TMG pairing: 500–1000 mg daily helps with methyl-donor demand on loading cycles longer than 4 weeks, or if low mood / brain fog shows up on NAD+.

For the research background, see NAD+ and aging research. Refrigerate after reconstitution and use within 4 weeks; keep the spray refrigerated and use within the timeframe on the bottle.

Disclaimer: All information presented in this article is for educational and informational purposes only. Platinum Biolabs does not promote or endorse the use of peptides for human consumption. All products sold by Platinum Biolabs are intended strictly for laboratory and research use only. Consult a qualified healthcare professional before making any health-related decisions.