HCG 5000IU: Reconstitution and Dosing in Research

Dosing Guide — 2026-10-03

A 5000IU HCG vial is a common size in hormonal and reproductive research, and reconstituting it correctly is what makes accurate measurement possible. Human chorionic gonadotropin (HCG) ships as a lyophilized powder; you add bacteriostatic water to put it into solution. This guide covers the step-by-step, the concentration and syringe-unit math for a 5000IU vial, and how many doses it provides. For laboratory research use only.

Platinum Biolabs ships every HCG vial with a batch-matched COA, from a US facility. Code PLATINUM25 takes 25% off your order at checkout.

What You Need

Your HCG 5000IU vial (lyophilized powder)
Bacteriostatic water for multi-use reconstitution
A sterile transfer syringe and a U-100 insulin syringe
Alcohol wipes

Step-by-Step

Bring the vial to room temperature; wipe the stopper with alcohol.
Draw your chosen volume of bacteriostatic water (see table).
Run the water slowly down the inside wall of the vial — HCG is a delicate glycoprotein, so avoid squirting directly onto the powder.
Do not shake. Swirl gently until dissolved into a clear solution.
Label and refrigerate immediately.

Concentration and Dose Math

How much water you add sets IU per syringe unit on a U-100 insulin syringe (100 units = 1 mL):

Bac water added	Concentration	Per unit (U-100)	500 IU dose	1000 IU dose
1 mL	5000 IU/mL	50 IU	10 units	20 units
2 mL	2500 IU/mL	25 IU	20 units	40 units

Adding 2 mL gives finer resolution for smaller research amounts; 1 mL keeps injection volume minimal. Choose whichever puts your target amount at an easy-to-read number of units.

How Many Doses in a 5000IU Vial

Research amount	Doses per 5000IU vial
500 IU	10
1000 IU	5
1500 IU	~3.3

See how long a 5000IU vial lasts for the full breakdown, and HCG in research for background on its LH-like activity.

Storage After Reconstitution

Refrigerate at 2–8°C — reconstituted HCG is sensitive and should be kept cold at all times
Generally used within about 30 days once reconstituted in bacteriostatic water
Protect from light and heat; do not freeze-thaw repeatedly
See the peptide storage guide

Bottom Line

Reconstitute a 5000IU HCG vial by running bacteriostatic water down the wall and swirling gently — never shaking. Your water volume sets the IU-per-unit math; 1 mL gives 50 IU/unit, 2 mL gives 25 IU/unit. A 5000IU vial yields roughly 5–10 common research doses. If your protocol needs more, compare the 10000IU vial.

Code PLATINUM25 takes 25% off at checkout. Every HCG 5000IU vial ships with a batch-matched COA.

Disclaimer: All information presented in this article is for educational and informational purposes only. Platinum Biolabs does not promote or endorse the use of peptides for human consumption. All products sold by Platinum Biolabs are intended strictly for laboratory and research use only. Consult a qualified healthcare professional before making any health-related decisions.