Wolverine Stack Dosing Protocol: The Complete Daily Schedule

Dosing Guide — 2026-11-06

A dosing *protocol* is more than a single number — it's the dose, the frequency, the route, the cycle length, and the vial math working together. This is the complete Wolverine Stack (BPC-157 + TB-500) protocol in one place, built on the same numbers as our full Wolverine Stack dosing guide and the Wolverine 10mg / Wolverine 20mg protocol pages.

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The protocol in one table

Parameter	Standard protocol
Dose	250 mcg BPC-157 + 250 mcg TB-500 (500 mcg blend)
Frequency	Once daily
Route	Subcutaneous
Units (10mg vial)	10 units
Units (20mg vial)	5 units
Reconstitution	2 mL bacteriostatic water
Cycle length	6–12 weeks
Off-cycle	2–4 weeks between runs

Step 1 — Reconstitution

Add 2 mL bacteriostatic water to either Wolverine vial. This gives:

Wolverine 10mg → 5 mg/mL → 1 unit = 50 mcg blend
Wolverine 20mg → 10 mg/mL → 1 unit = 100 mcg blend

Inject the water slowly down the vial wall, swirl gently, do not shake, and refrigerate. Full technique is in the reconstitution guide.

Step 2 — Daily dose

Draw the standard 500 mcg blend per day:

Wolverine 10mg: 10 units
Wolverine 20mg: 5 units

For acute-injury research, the aggressive variation doubles this to 1000 mcg/day (20 units on the 10mg vial, 10 units on the 20mg vial). Full per-day and per-week breakdowns are in dosage per day and dosage per week.

Step 3 — Injection schedule and site rotation

Once daily, same time each day for consistency.
Subcutaneous into pinched abdominal fat or thigh.
Rotate sites every day to avoid local irritation.
For tendon/ligament research, inject the BPC-dominant blend near the target tissue — localized proximity matters for BPC-157. TB-500 acts systemically, so its site is less critical.

Step 4 — Cycle length

Research endpoint	Cycle
Acute injury	4 weeks on, then assess
General recovery	6 weeks on, 4 weeks off
Tendon/ligament	8–12 weeks on, 6 weeks off
Chronic GI (BPC-dominant)	8 weeks on, 4 weeks off

Unlike GH-axis peptides, Wolverine has minimal receptor-desensitization concern, so extended cycles are well-tolerated. Off-weeks are mainly for confirming endpoint stability.

Step 5 — Vial selection and cost

Vial	Days at standard dose	Best for
Wolverine 10mg	~20 days	Short runs, testing tolerance
Wolverine 20mg	~40 days (~5–6 weeks)	Full cycles — most economical

A 6-week cycle uses about 2 vials of Wolverine 10mg or 1 vial of Wolverine 20mg. For the comparison between sizes, see Wolverine 10mg vs 20mg.

Optional: the GHK-Cu add-on

The Wolverine Repair Stack adds GHK-Cu for matrix-remodeling and collagen-synthesis signaling on top of the BPC + TB protocol — run GHK-Cu ~2 mg once daily alongside the standard Wolverine dose over a 6–8 week cycle. Details are in the full dosing guide.

Disclaimer: All information presented in this article is for educational and informational purposes only. Platinum Biolabs does not promote or endorse the use of peptides for human consumption. All products sold by Platinum Biolabs are intended strictly for laboratory and research use only. Consult a qualified healthcare professional before making any health-related decisions.